Coding
Part:BBa_K1808005:Design
Designed by: Remsha Afzal Group: iGEM15_TrinityCollegeDublin (2015-09-16)
ADH1 HISTAG from artemisia annua 6 His tagged
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 895
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.
Source
Artemisia annua